Biodegradation Predictive Worksheet Reasoning: Tetracyanoethylene

1. What was the first thing you focused on to guide your development of a pathway(s)?
E1 The cyano groups can undergo hydrolysis to yield carboxylic acids. Anaerobically, tetracyanoethylene can serve as a final electron acceptor analogous to tetrachloroethylene. The cyano groups can get reduced to a methylene carbon and an amino group. The cyano group might behave as a pseudo halide
E2 Detoxify cyanogen via nitrile hydratase.
E3 May be very toxic to bugs!
Answer to questions of the check list:
question 1: yes
question 2: yes
question 4: no
question 5: yes (must exhibit high redox potential: currently checked as substrate for PCE dehalogenase!)
E4 Looks like PCE --- make similar mechanism

2. How did you proceed after your initial focus point?
E1 The latter possibility above would be analogous to tetrachloroethylene. After 3 consecutive 2 electron reductions, acrylonitrile is obtained and its metabolism is described in the UM-BBD.
E2 -
E3 answers to questions of the check list:
question 6: no
question 7: no
question 8a: yes
E4 similar mechanism as PCE